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KMID : 0378019750180081049
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New Medical Journal
1975 Volume.18 No. 8 p.1049 ~ p.1060
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Studies on Classification, Pathogenicity and Fastidiousness of Mycobacteria Isolated from Soil
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金聖光/Kim, Sung-Kwang
崔大卿/柳駿/Choi, Tai-Kyung/Lew, Joon
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Abstract
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Acid-fast organisms were isolated from 120 soil samples collected at four villages in the country, - Tests were conducted to determine the biological and biochemical characteristics and the fastidiousness of the isolated mycobacteria. The pathogenicity of the acid-fast organisms isolated from soil were compared with the pathogenicity of reference strains. The results are summarized as follows:
1. Of the 120 samples cultured, 37(30.8%) yielded atypical mycobacteria.
2. Out of the 37 strains isolated 7 strains would not grow in subculture showing their fastidiousness. Although these seven mycobacterial strains grew well in the primary isolation, they did . not grow in subculture under the same conditions as in their primary isolation, nor did they grow on ordinary laboratory culture media.
3. The isolated strains, when classified by Runyon groups, consisted of 5 strains (16.6%) of scotochromogens, 3 strains (10.0%) of non-photochromogens and 22 strains (73.4%) of rapid growers.
4. According to the biochemical characteristics, the 5 strains of scotochromogen could be subdivided into 2 groups, the 3 strains of non-photochromogen into 3 groups and the 22strains of rapid grower
into 6 groups.
5. Acid-fast organisms were observed in liver and spleen of mice inoculated intraperitoneally with three of the strains of rapid grower isolated from soil and in mice inoculated with the reference strain, M. fortuitum, by 3 weeks after inoculation, but no acid-fast organisms were observed in the organs of the mice inoculated with the isolated scotochromogens and non-photochro
mogens by 4 weeks.
6. In the histopathological examinations, early granulomatous lesions with acid-fast organisms were observed only in the liver tissue.
The fastidiousness of 7 strains of mycobacteria from soil were very similar to the fastidiousness of M. leprae which is still unculturable although there have often been claims to have grown the organisms in primary isolation. Further study on the mycobacterial fastidiousness is needed in the future.
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